Fig. 3
From: Role of Rab5 in the formation of macrophage-derived foam cell
Down-regulation of Rab5 in U937-derived macrophages inhibited the formation of foam cells. a U937-derived macrophages were lysed and analyzed by qRT-PCR for Rab5 mRNA after transfection with the indicated siRNA (n = 2, *P < 0.05; ***P < 0.001). b Time course study was carried out to evaluate the expression of Rab5 mRNA followed by the transfection of siRab5–775 (n = 3, *P < 0.05; ***P < 0.001 vs. 0 h control group). c Efficiency of knock-down of Rab5 protein by siRab5–775 was analyzed by western blot. d U937-derived macrophages transfected with the indicated siRNAs for 48 h, incubated for 48 h with 80 μg/ml oxLDL, and stained with oil red O. Scale bars = 20 μm. e Quantification of TC and CE of U937-derived macrophages treated with indicated siRNAs and 80 μg/ml oxLDL (n = 3, *P < 0.05; **P < 0.01)