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Fig. 2 | Lipids in Health and Disease

Fig. 2

From: Identification of deleterious rare variants in MTTP, PNPLA3, and TM6SF2 in Japanese males and association studies with NAFLD

Fig. 2

Localization of GFP-tagged PNPLA3 in Huh-7 cells. a Huh-7 cells grown on chamber slides were transfected with pEGFP-PNPLA3 encoding each variation labeled on the left. Four hours after the transfection, medium was changed to fresh medium supplemented with 200 μM oleic acid. After incubation for 24 h with oleic acid, Huh-7 cells were fixed and stained with LipidTOX Red and DAPI. The stained cells were visualized under a fluorescence microscope. Yellow scale bars indicate 10 μm. b The ratio (%) of GFP-PNPLA3 on lipid droplets to total GFP fluorescence and (c) LipidTOX fluorescence intensity in GFP-expressing cells were measured from 10 randomly selected fields. The average value and standard deviations are shown. *P < 0.05 (Student’s t-test)

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