Fig. 4

MTP activities of MTTP variants. a MTTP cDNAs encoding the indicated rare variants were inserted into the pCR3 vector. The constructs were transfected into COS-7 cells, and 24 h after the transfection, cells were harvested and homogenized by sonication. Each homogenate containing 100 μg protein was used for triplicate MTP lipid transfer assays. Average fluorescence with standard deviations are shown. Statistical significance compared to wild-type MTTP is indicated. *P < 0.05, Student’s t-test. b Remaining homogenate containing 20 μg protein was used for western blotting with anti-MTTP or anti-actin