Fig. 2

Effect of SDA on 3T3-L1 adipocyte viability. Two-day post-confluency preadipocytes were incubated with differentiation medium in the presence of SDA (0, 200, and 400 μM) for 24 or 72 h. Cell viability was detected by calcein-AM/propidium iodide staining. a A representative image of cell staining photographed using a microscope (X200). Living cells were stained with green (calcein staining) and dead cells were stained with red (propidium iodide staining). b Quantification of cell viability using spectrophotometry. Three images for each treatment were captured and analyzed. Values were obtained from three independent experiments and are expressed as the means ± SD; different from control cells: ^* P < 0.05