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Fig. 4 | Lipids in Health and Disease

Fig. 4

From: Suppression of adipocyte differentiation and lipid accumulation by stearidonic acid (SDA) in 3T3-L1 cells

Fig. 4

Effect of SDA on mRNA expression levels of adipogenic transcription factors and genes in 3T3-L1 adipocytes. Two-day post-confluency preadipocytes were incubated with differentiation medium in the presence of SDA (0, 50, and 200 μM). a The expression of C/EBPα, C/EBPβP, PPARγ, and SREBP-1c was evaluated by qRT-PCR with specific primer pairs on Day 3. b The expression of AP2, FAS, SCD-1, LPL, GLUT4, and PEPCK was evaluated by qRT-PCR on Day 6. The relative qRT-PCR values were corrected to 36B4 expression levels and normalized with respect to the control. Data were obtained from three independent experiments and are expressed as mean ± SD; different from control cells: *P < 0.05

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Lipids in Health and Disease

ISSN: 1476-511X