Fig. 5From: Suppression of adipocyte differentiation and lipid accumulation by stearidonic acid (SDA) in 3T3-L1 cellsPPARγ binding activity in 3T3-L1 adipocytes treated with SDA. Two-day post-confluency preadipocytes were incubated with differentiation medium in the presence of SDA (0 and 200 μM) for 24, 48, and 72 h. ELISA-based PPARγ transcription factor assay was conducted to detect PPARγ binding activity to PPRE-containing double-stranded DNA sequences. Data were obtained from three independent experiments. Values are expressed as mean ± SD; different from control cells: *P < 0.05Back to article page