Fig. 3From: Oleate ameliorates palmitate-induced reduction of NAMPT activity and NAD levels in primary human hepatocytes and hepatocarcinoma cellsNMN was able to restore reduced NAD concentrations but not cell viability after stimulation with palmitate. NAMPT activity and NAD levels were not involved in hepatic triglyceride storage. a HepG2 cells were either stimulated with palmitate (0.5 mM), the specific NAMPT inhibitor FK866 (10 nM), NMN (0.5 mM) or with the indicated combinations for 48 h. Cell viability and b NAD concentration were measured using WST-1 assay and EnzyChrom™ NAD+/NADH Assay Kit (E2ND-100), respectively. c HepG2 cells were stimulated with palmitate (0.5 mM), oleate (1 mM), a combination of both fatty acids (0.5 mM/1 mM), FK866 (10 nM) or with a combination of oleate and FK866. Oil red O staining was performed. Data were normalized to control (serum free medium) which was set 1 and represent three independent experiments performed in triplicates shown as means ± SEM. *p < 0.05, **p < 0.01 compared to control. # p < 0.05, ## p < 0.01 compared to equivalent control (palmitate or FK866)Back to article page