Fig. 3

HFD-induced T2DM mice treated with fenofibrate down regulated the expression of osteoblast extracellular matrix. a Immunohistochemical staining for Collagen I in representative sections of femur from different groups. Magnification was 100×. b Area fraction of collagen I was counted from 5 slides (10 fields in each slide). c, d Western blot analysis for Collagen I and osteocalcin protein expression extracted from the bones of different groups. β-actin was used as control. ^* P < 0.05, ^** P < 0.01, ^*** P < 0.001