Table 1 Primer sequences used for quantitative PCR
Primer | Forward (5′–3′) | Reverse (5′–3′) |
|---|---|---|
Runx2 | AACGATCTGAGATTTGTGGGC | CCTGCGTGGGATTTCTTGGTT |
Osteocalcin | CTGACCTCACAGATCCCAAGC | TGGTCTGATAGCTCGTCACAAG |
Spp1 | AGCAAGAAACTCTTCCAAGCAA | GTGAGATTCGTCAGATTCATCCG |
Sp7 | ATGGCGTCCTCTCTGCTTG | TGAAAGGTCAGCGTATGGCTT |
Bcl2 | GTCGCTACCGTCGTGACTTC | CAGACATGCACCTACCCAGC |
Caspase3 | ATGGAGAACAACAAAACCTCAGT | TTGCTCCCATGTATGGTCTTTAC |
β-actin | CATGTACGTTGCTATCCAGGC | CTCCTTAATGTCACGCACGAT |