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Fig. 2 | Lipids in Health and Disease

Fig. 2

From: Hepatitis B virus inhibits the in vivo and in vitro synthesis and secretion of apolipoprotein C3

Fig. 2

Effect of pHBV1.3 on the promoter activity and mRNA and protein expression levels of ApoC3. a At 48 h after 0.6 μg of pHBV1.3 and its control plasmid pBlue-ks were each co-transfected with 0.2 μg of ApoC3 gene promoter pApoC3-Luc into HepG2 cells, the changes in luciferase activity were determined using a luminometer; each experiment was repeated three times; b at 48 h after 4 μg of pHBV1.3 and its control plasmid pBlue-ks were each transfected into HepG2 cells, the effect of HBV on the expression of ApoC3 mRNA was determined by RT-qPCR; c The expression levels of ApoC3 protein in HepG2 and HepG2.2.15 cells were determined by Western blot; d The content of ApoC3 in the supernatant of HepG2 cells was determined by ELISA. *P < 0.005

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