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Fig. 3 | Lipids in Health and Disease

Fig. 3

From: Quantification of sterol-specific response in human macrophages using automated imaged-based analysis

Fig. 3

Two-channel fluorescence images of THP-1 derived macrophages exposed to various conditions. Nuclei were stained with DAPI (blue), lipids were stained with Nile Red (red), and cells were imaged at 20× magnification. Scale bar is 20 μm. -ApoB (pre-cultured with rabbit anti-apolipoprotein B antibodies), Chol (cholesterol and 1-palmitoyl-lyso-phosphatidylcholine (LPC) in PBS), CHS (cholesteryl hemisuccinate and LPC in PBS), Erg (ergosterol and LPC in PBS), LDL (low-density lipoprotein in PBS), LPS (lipopolysaccharide in PBS), PBS (phosphate-buffered saline vehicle), Ser (medium contained 10% fetal bovine serum)

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Lipids in Health and Disease

ISSN: 1476-511X