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Table 2 Activated and inhibited upstream regulators predicted by IPA following 2 cycles of chemotherapy

From: Chemotherapy diminishes lipid storage capacity of adipose tissue in a preclinical model of colon cancer

Upstream regulator Molecular type Predicted activation state Activation Z-score* P-value of overlap Target molecules in differentially expressed dataset
PPARGC1A Transcription regulator Inhibited −4.379 2.17E-19 ACACA, ACADVL, ACAT1, ATP5O, CAT, COX5A, CS,DLAT, FASN, IDH3A, MDH2, ME1, MT-CO2, NDUFV2, PCK1, PDHA1, PGAM1, PRDX3, SLC2A4,UQCRFS1
PPARγ Ligand-dependent nuclear receptor Inhibited −4.06 1.629E-23 ACAA1, ACACA, ACLY, ATP5O, CAT, CPT2, CS, DLAT, FABP1, FASN, FDPS, GAPDH, GPD1,GPT, IDH1, IVD, KRT18, LIPE, ME1, MGLL, PC, PCK1, PDHB, PYGL, RPSA, SCP2, Slc25a1, SLC2, TKT
PPARα Ligand-dependent nuclear receptor Inhibited −2.97 4.41E-16 ACAA1, ACACA, ACADVL, ACAT1, ACAT2, CAT, CPT2, CS, DBI, FABP1, FASN, FDPS, GPD1, GPT, MGLL,MT-CO2, PC, PCK1, PRDX6, SCP2, SLC2A4, UQCRC1
MYC Transcription regulator Inhibited −2.61 2.27E-12 ACACA, ACAT1, AK2, ALDOA, CANX, CAPNS1, CPT2, DBI, ENO1, FABP1, FASN, GAPDH, GCSH, GPT, HSPE1, IDH1, IDH2, LUM, NDRG2, PCK1, PDHA1, PGAM1, PHB, PHB2, PRDX3, SLC25A5, TKT
SREBF1 Transcription regulator Inhibited −2.13 7.82E-09 ACACA, ACLY, ALDOC, DBI, FASN, FDPS, GPX3, IDH1, PCK1, THRSP, UQCRFS1
MAP4K4 Kinase Activated 3.44 8.13 E-13 ACACA, ACADVL, ACLY, DLAT, FASN, IVD, MGLL, PGAM1, SCP2, SLC2A4, UQCRC1, UQCRFS1
5-Fluorouracil Chemical drug Activated 3.21 1.30E-11 ALDOA, ATP5O, CANX, CAPNS1, ECHS1, FDPS, GAPDH, HSPE1, IDH2, NDUFV2, PSMA7, RPS8, SLC25A5, UQCRC2
  1. *Z > 2 and Z < −2 predict activation and inhibition of the upstream regulator, respectively. The p-value indicates the significance of the overlap between the molecules targeted by the upstream regulator in the IPA database and the experimental dataset. Abbreviations: PPARγ, Peroxisome proliferator-activated receptor gamma; PPARα, Peroxisome proliferator-activated receptor alpha; PPARGC1A, Peroxisome proliferator-activated receptor gamma coactivator 1-alpha; SREBF1 (SREBP1c), Sterol regulatory element binding transcription factor 1; MAP4K4, Mitogen-activated protein kinase kinase kinase kinas