Fig. 2

Female sex increases antioxidant protein expression. Quantitation of Ref-1 (a), γ-GCS (b), SOD-1 (c), and SOD-2 (d) protein abundance in the liver, normalized to the Ponceau stain, and further normalized to the adult, male control group. Top panels: representative immunoblots for target protein expression and Ponceau-stained membranes. Bottom panels: quantitation of target protein expression. Results are expressed as means + S.E.M., n = 5–7 animals per group. † Significant main effect of sex. * Significant effect of aging within a diet and sex group. AC: adult control diet; AT: adult trans-fat diet; OC: old control diet; OT: old trans-fat diet