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Fig. 3 | Lipids in Health and Disease

Fig. 3

From: Acute administration of beta-caryophyllene prevents endocannabinoid system activation during transient common carotid artery occlusion and reperfusion

Fig. 3

Western blot analysis of CB1 (a, b), CB2 (c, d), peroxisome-proliferator activated receptor-alpha (PPAR-alpha) (e, f) and cyclooxygenase-2 (COX-2) (g, h) in the frontal cortex of sham and bilateral common carotid artery occlusion followed by reperfusion (BCCAO/R) vehicle and BCP-pre-treated rats. b, d: densitometric analysis of the band gray levels expressed as a percentage of the optical density (O.D.) ratio of bands immunostained for CB1, CB2, PPAR-alpha and COX-2 to those of GAPDH. Data are reported as mean values of 12 sham-operated+vehicle, 12 BCCAO/R+ vehicle rats, 10 BCP-pre-treated sham-operated and 10 BCP-pre-treated BCCAO/R rats. Error bars depict the standard error of mean (S.E.M.). Asterisks denote significant differences. Two-way ANOVA with the Bonferroni’s test for post-hoc analyses was applied to evaluate statistical differences between groups. P values < 0.05 were considered significant (refer to Table 1 for F and p values relevant to effects of BCCAO/R and BCP treatment and to interaction between them)

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