Effects of Omegaven®, EPA, DHA and oxaliplatin on oesophageal adenocarcinoma cell lines growth, cytokine and cell signal biomarkers expression

Eltweri, Amar M.; Howells, Lynne M.; Thomas, Anne L.; Dennison, Ashley R.; Bowrey, David J.

doi:10.1186/s12944-018-0664-1

Lipids in Health and Disease

Table 1 Effects of EPA, DHA, Omegaven® and oxaliplatin treatments over time on OE33 and OE19 cell line expression of IL-6 and TNF alpha

From: Effects of Omegaven®, EPA, DHA and oxaliplatin on oesophageal adenocarcinoma cell lines growth, cytokine and cell signal biomarkers expression

			OE33 cell lines					OE19 cell lines
			Control	10 μM	Sign.	50 μM	Sign.	Control	10 μM	Sign.	50 μM	Sign.
IL-6	EPA	72 h	3.7 ± 0.33	4.6 ± 0.37	P = 0.12	4.3 ± 0.02	P = 0.18	3.8 ± 0.35	3.6 ± 0.53	P = 0.68	2.9 ± 0.13	P = 0.06
	EPA	144 h	3.0 ± 0.38	3.6 ± 0.22	P = 0.18	5.1 ± 0.17	*P = 0.03*	2.7 ± 0.20	3.2 ± 0.26	P = 0.13	3.6 ± 0.06	*P = 0.03*
	DHA	72 h	4.5 ± 0.37	4.8 ± 0.51	P = 0.60	4.5 ± 0.11	P = 0.87	4.1 ± 0.35	3.7 ± 0.32	P = 0.43	3.7 ± 0.13	P = 0.32
	DHA	144 h	5.6 ± 0.87	6.2 ± 0.02	P = 0.05	5.1 ± 0.38	P = 0.29	3.9 ± 0.45	4.5 ± 0.06	P = 0.21	4.3 ± 0.23	P = 0.37
	Omegaven®	72 h	4.7 ± 0.20	5.6 ± 0.18	*P = 0.03*	5.6 ± 0.24	*P = 0.04*	3.8 ± 0.05	4.0 ± 0.41	P = 0.60	4.7 ± 0.49	P = 0.09
	Omegaven®	144 h	5.4 ± 0.15	5.2 ± 0.27	P = 0.32	4.9 ± 0.30	P = 0.13	3.1 ± 0.14	4.0 ± 0.27	P = 0.05	4.7 ± 0.07	*P = 0.007*
	Oxaliplatin	72 h	4.9 ± 0.11	5.7 ± 0.15	*P = 0.01*	4.8 ± 0.11	P = 0.18	4.0 ± 0.15	4.5 ± 0.44	P = 0.30	4.1 ± 0.73	P = 0.97
	Oxaliplatin	144 h	4.4 ± 0.00	4.1 ± 0.39	P = 0.46	4.7 ± 0.01	P = 0.08	3.7 ± 0.54	4.7 ± 0.03	P = 0.15	5.8 ± 0.66	P = 0.07
TNF alpha	EPA	72 h	2.6 ± 0.55	3.3 ± 0.45	P = 0.29	2.7 ± 0.27	P = 0.82	2.3 ± 0.12	2.2 ± 0.31	P = 0.78	2.2 ± 0.13	P = 0.42
	EPA	144 h	2.1 ± 0.40	2.5 ± 0.05	P = 0.28	3.6 ± 0.23	P = 0.06	1.6 ± 0.14	2.1 ± 0.24	P = 0.14	2.5 ± 0.13	*P = 0.02*
	DHA	72 h	3.3 ± 0.12	3.9 ± 0.38	P = 0.85	3.0 ± 0.49	P = 0.42	2.2 ± 0.06	2.3 ± 0.12	P = 0.40	2.7 ± 0.11	*P = 0.03*
	DHA	144 h	3.8 ± 0.22	4.4 ± 0.13	P = 0.08	3.6 ± 0.20	P = 0.48	2.3 ± 0.13	2.8 ± 0.00	*P = 0.04*	2.9 ± 0.09	*P = 0.04*
	Omegaven®	72 h	3.2 ± 0.05	3.6 ± 0.21	P = 0.06	3.7 ± 0.02	*P = 0.008*	2.2 ± 0.08	2.5 ± 0.20	P = 0.20	3.3 ± 0.39	*P = 0.04*
	Omegaven®	144 h	3.6 ± 0.05	3.3 ± 0.33	P = 0.34	2.7 ± 0.30	P = 0.05	2.2 ± 0.00	2.6 ± 0.28	P = 0.17	3.3 ± 0.03	*P < 0.001*
	Oxaliplatin	72 h	3.3 ± 0.08	4.1 ± 0.20	P = 0.03	3.3 ± 0.06	P = 0.99	2.5 ± 0.12	2.8 ± 0.20	P = 0.23	2.9 ± 0.39	P = 0.40
	Oxaliplatin	144 h	2.6 ± 0.00	2.6 ± 0.01	P = 0.03	2.9 ± 0.19	P = 0.11	2.3 ± 0.34	3.1 ± 0.09	P = 0.14	4.1 ± 0.88	P = 0.10

Data presented as mean ± SD (pg/ml), two tailed student t test was used to identify any significance of log 10 transformed data, P < 0.05 considered significant. Bold text indicate significant upregulation compared to control

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