Fig. 2

The effects of fatty acids on cholesterol uptake in Caco-2 monolayer. Caco-2 cells were incubated for 21 days, and the cholesterol uptake of 60 min and 120 min was analyzed as described under the Materials and Methods. Micellar solutions with 0.1% (v/v) DMSO were as follows: 2 μCi/mL [1,2-³H (N)]-cholesterol, 100 μmol/L cholesterol, 1 mmol/L OLA (for Control) or 0.5 mmol/L indicated fatty acid (PAM, OLA, LNA, ARA, EPA and DHA), 0.5 mmol/L monoolein, 6.6 mmol/L sodium taurocholate, and 0.1 mmol/L soy PtdCho in cultural medium containing the delipidized FBS. Data were represented as mean ± SD of three independent experiments (a > b > c > d, A > B > C > D > E, all p < 0.01). PAM: palmitic acid; OLA: oleic acid; LNA: linoleic acid; ARA: arachidonic acid; EPA: eicosapentaenoic acid; DHA: docosahexaenoic acid