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Table 3 Relationship between alkaline phosphatase activity and culture time of Caco-2 monolayera

From: Fatty acids modulate the expression levels of key proteins for cholesterol absorption in Caco-2 monolayer

t/d

Ratio of alkaline phosphatase

Activity (U/g protein)

5

1.33 ± 0.04c

21.83 ± 2.55b

9

2.76 ± 0.11c

21.15 ± 2.14b

13

5.35 ± 0.34c

18.70 ± 1.64b

17

12.15 ± 0.65b

57.25 ± 4.46a

21

15.65 ± 0.79a

65.79 ± 6.34a

  1. a Alkaline phosphatase activity of the Caco-2 monolayer was measured using the Alkaline Phosphatase Activity Assay Kit (CBA-301, Cell Biolabs, Inc., CA, USA). Each value was represented as mean ± SD of three independent experiments (a > b > c > d, all p < 0.01)