Fig. 2

Rab8a phosphorylation promotes lipid droplets enlargement. 3 T3-L1 pre-adipocytes were transfected with MYC-LRRK2 (Wild type or Y1699C), FLAG-Rab8a (WT or T72A mutant) and Fsp27-Cherry. Oleic acid was added to promote the formation of LDs for 15 h. LDs were labeled with Bodipy 493/503 (Green). a Expression levels of LRRK2, Rab8a and Fsp27 were detected by western blot. b Co-expression of LRRK2 Y1699C with FLAG-Rab8a increases the sizes of LDs when over-expressing Fsp27. Scale bars represent 20 μm. c-d Statistical analysis of (b). Significance was established using a two-tailed Student’s t-test. Differences were considered significant at P < 0.05. ***P < 0.001