Fig. 4

Phosphorylation of Rab8a weakens its interaction with its effector Optineurin. Two hundred ninety-three T cells were transfected with FLAG-Rab8a (WT, T72A, T72D, T72E, T22 N, T22 N/T72A, T22 N/T72D) and HA-Optineurin. Anti-FLAG M2 beads were used for immunoprecipitation. The immunoprecipitated products were detected by using antibodies against FLAG or HA. IP, immunoprecipitation; IB, immunoblot; Western blots showing protein level of HA-Optineurin (top panel) and FLAG-Rab8a (second panel from the top) in the IP products. Western blots showing protein level of HA-Optineurin (third panel from the top) and FLAG-Rab8a (bottom panel) in the total cell lysis