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Fig. 2 | Lipids in Health and Disease

Fig. 2

From: Proteasome inhibitors modulate anticancer and anti-proliferative properties via NF-kB signaling, and ubiquitin-proteasome pathways in cancer cell lines of different organs

Fig. 2

(1–4) Dose-dependent response for anti-proliferative properties of various compounds in cancer cells of Hela-1, liver-2, pancreas-3, and prostate-4. The cancer cell lines of Hela, liver, pancreas, and prostate were maintained in DMEM supplemented with 10% heat inactivated FBS and 10 mg/mL, gentamicin at 37 °C in a humidified atmosphere with 5% carbon dioxide (CO2) and 95% oxygen (O2) as described previously [17]. Cancer cells (1 × 105) of various organs were seeded in 48 well tissue culture plate with 900 μl of medium containing 0.2% dimethyl sulfoxide of different types of cancer cell lines (Hela cell, liver, pancreas, and prostate), and incubated at 37 °C for 2 h. After 2 h, different concentrations (100 μl of 2.5, 5, 10, 20, 40, or 80 μM) of thiostrepton, ampicillin, dexamethasone, 2-methoxyestradiol, δ-tocotrienol, (−) riboflavin, ascorbic acid, quercetin, amiloride, and quinine sulphate in triplicate were added to each well, incubated for 48 h at 37 °C in a humidified atmosphere of 5% CO2. Followed by counting of live cells of each well by trypan blue dye exclusion or a quantitative colorimetric assay with 3-(4, 5)-dimethylthiozol-2, 5-diphenyl-tetrazolium bromide (MTT), as described previously [18, 19]. The anticancer properties and dose-dependence for eleven compounds are presented for Hela, liver, pancreas, and prostate cancer cell lines. Values in a column not sharing a common symbol are significantly different at ¶ = P < 0.001; § = P < 0.01; ‡ = P < 0.05; † = control

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