Fig. 3

(5–8) Dose-dependent response for anti-proliferative properties of various compounds in cancer cell lines of breast-5, lung-6, Melanoma-7, and B-lymphocytes-8. The cancer cell lines of breast, lung, melanoma, and B-lymphocytes were maintained in DMEM supplemented with 10% heat inactivated FBS and 10 mg/mL, gentamicin at 37 °C in a humidified atmosphere with 5% carbon dioxide (CO₂) and 95% oxygen (O₂) as described previously [17]. Cancer cells (1 × 10⁵) of various organs were seeded in 48 well tissue culture plate with 900 μl of medium containing 0.2% dimethyl sulfoxide of different type of cancer cell lines (breast, lung, melanoma, and B-lymphocytes), and incubated at 37 °C for 2 h. After 2 h, different concentrations (100 μl of 2.5, 5, 10, 20, 40, or 80 μM) of thiostrepton, ampicillin, dexamethasone, 2-methoxyestradiol, δ-tocotrienol, (−) riboflavin, ascorbic acid, quercetin, amiloride, and quinine sulphate in triplicate added in each well, and then incubated for 48 h at 37 °C in a humidified atmosphere of 5% CO₂. Followed by counting the living cells of each well by trypan blue dye exclusion or a quantitative colorimetric assay with 3-(4, 5)-dimethylthiozol-2, 5-diphenyl-tetrazolium bromide (MTT) as described previously [18, 19]. The anticancer properties as dose-dependent of eleven compounds presented for breast, lung, melanoma, and B-lymphocytes cancer cell lines. Values in a column not sharing a common symbol are significantly different at ¶ = P < 0.001; § = P < 0.01; ‡ = P < 0.05; † = control