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Fig. 4 | Lipids in Health and Disease

Fig. 4

From: Proteasome inhibitors modulate anticancer and anti-proliferative properties via NF-kB signaling, and ubiquitin-proteasome pathways in cancer cell lines of different organs

Fig. 4

(9) Dose-dependent response for anti-proliferative properties of various compounds in T-cells (Jurkat). The T-cells (Jurkat) maintained in DMEM supplemented with 10% heat inactivated FBS and 10 mg/mL, gentamicin at 37 °C in a humidified atmosphere with 5% carbon dioxide (CO2) and 95% oxygen (O2) as described previously [17]. The T-cells (1 × 105) of was seeded in 48 well tissue culture plate with 900 μl of medium, containing 0.2% dimethyl sulfoxide of T-cells (Jurkat), and incubated at 37 °C for 2 h. After 2 h, different concentrations (100 μl of 2.5, 5, 10, 20, 40, or 80 μM) of thiostrepton, ampicillin, dexamethasone, 2-methoxyestradiol, δ-tocotrienol, (−) riboflavin, ascorbic acid, quercetin, amiloride, and quinine sulphate in triplicate added in each well, and then incubated for 48 h at 37 °C, in a humidified atmosphere of 5% CO2. Followed by counting the living cells of each well by trypan blue dye exclusion or a quantitative colorimetric assay with 3-(4, 5)-dimethylthiozol-2, 5-diphenyl-tetrazolium bromide (MTT) as described previously [18, 19]. The anti-proliferation properties as dose-dependent with eleven compounds were presented in T-cells (Jurkat). Values in a column not sharing a common symbol are significantly different at ¶ = P < 0.001; § = P < 0.01; ‡ = P < 0.05; † = control

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