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Fig. 8 | Lipids in Health and Disease

Fig. 8

From: Proteasome inhibitors modulate anticancer and anti-proliferative properties via NF-kB signaling, and ubiquitin-proteasome pathways in cancer cell lines of different organs

Fig. 8

a-d The expression of important inflammatory biomarkers and proteasome subunits of total RNAs obtained from plasma after EDTA treated whole blood after feeding δ-tocotrienol (500 mg/d) for 6-weeks to hepatitis C patients. The quantitative Real-time qRT-PCR was performed on total mRNA isolated from plasma samples of hepatitis C patients of pre-dose and post-dose samples. Taqman primer-probes and Step-one qRT-PCR kit were used for these estimations. All reactions were performed in triplicate using equal amounts of total RNA per reaction. Reverse-transcriptase step involved incubation at 48 °C for 15 min. The PCR cycling conditions included an initial denaturation at 95 °C for 10 min, followed by 40 cycles of 95 °C for 15 s, and 60 °C for 60 s. Real-time PCR assays were completed using a Step-one plus Real time PCR system. Relative gene expression of hepatitis C patients of pre-dose vs post-dose samples were normalized (2−ΔΔCT analysis) to GAPDH. Values in a column not sharing a common symbol are significantly different at ¶ = P < 0.001; § = P < 0.01; ‡ = P < 0.05; † = control

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