Fig. 2

Palmitate reduces the expression of the Slc2a4 gene in L6 myotubes. L6 myotubes were treated with 0.5% ethanol (vehicle) for 12 h (control sample, C) or 0.75 mM palmitate for 2, 6 or 12 h. a Relative Slc2a4 mRNA normalized by Gapdh mRNA (RT-qPCR). b Relative GLUT4 protein normalized by total protein analysis with Coomassie Blue-stained gel (Western blotting). Values are means ± SEM of 6 to 10 samples; *P < 0.05 and **P < 0.01 versus C; one-way ANOVA, Student–Newman–Keuls as post hoc test