Skip to main content
Fig. 2 | Lipids in Health and Disease

Fig. 2

From: Heat shock protein 70 promotes lipogenesis in HepG2 cells

Fig. 2

HSP70 overexpression induces fat accumulation and increases the size of lipid droplets. Construction of Lenti-HSPA1A lentiviral vectors for identification of efficient lenti-HSPA1A lentivirus. Schematic representation of lentiviral construction (a), HSP70 protein (b) expression levels were evaluated by Western blot and normalized to GAPDH levels (c). Data are expressed as the mean ± SD. HepG2 cells infected with control or Hsp70-overexpressing lentivirus were treated with or without 0.5 mM PA for 24 h, and lipids were stained with Oil Red O and analysed by microscopy (d). Hsp70 overexpression had a small effect on lipid accumulation in HepG2 cells. The cells were infected with an Hsp70-overexpressing lentivirus and then treated with or without PA (0.5 mM) for 24 h. All experiments were repeated three times

Back to article page