Fig. 1

Adipogenesis of hADSCs induced by oleic acid (OA) in combination with insulin and dexamethasone. Cells were exposed to various concentrations of OA (50, 80, and 100 μM) or IBMX in combination with insulin and dexamethasone for 10 days. a Intracellular lipids were visualized by oil red O staining at day 10. b Quantification of intracellular lipids at the end of the incubation period (day 10). Briefly, oil red O-stained lipids were extracted with isopropanol, and the absorbance at 500 nm was determined by spectrophotometry. c and d The PPARγ protein expression level was analyzed by western blot at day 10. Data are shown as the mean ± SD (n = 3). **p < 0.01 vs. control, ^##p < 0.01 vs. IBMX