Fig. 1
From: A20 deubiquitinase controls PGC-1α expression in the adipose tissue
PGC-1α and A20 expression in the adipose tissue of humans. Samples containing 500 μg total protein from abdominal subcutaneous adipose tissue specimens, collected from 9 lean volunteers and 12 obese subjects during a Roux-in-Y gastric bypass (BS) were used in immunoprecipitation experiments employing anti-PGC-1α (a, b, d and e) or anti-A20 (a and c) as primary antibodies. The immunoprecipitation procedure used antibodies sufficient to immunodeplete the sample. The immunoprecipitates were separated by SDS-PAGE, transferred to nitrocellulose membranes and blotted with anti-PGC-1α (a and b), anti-A20 (a, c and e) or anti-ubiquitin (a and d) antibodies. In A, typical blots are depicted; in B-E the quantification of bands is graphically represented. *p < 0.05 vs. lean