Fig. 4
From: A20 deubiquitinase controls PGC-1α expression in the adipose tissue
PGC-1α association with ubiquitin and A20 in distinct adipose tissue depots of mice. Samples containing 500 μg total protein from subcutaneous (SC), visceral (VI) or brown (BAT) adipose tissue depots from lean or obese mice were used in immunoprecipitation experiments employing anti-PGC-1α as primary antibody. The immunoprecipitation procedure used antibodies sufficient to immunodeplete the sample. The immunoprecipitates were separated by SDS-PAGE, transferred to nitrocellulose membranes and blotted with anti-ubiquitin (a-d) or anti-A20 (e-h) antibodies. In a and e, typical blots are depicted; in b-d and f-h the quantification of bands is graphically represented. In all experiments n = 5–6, *p < 0.05 vs. lean