Table 1 Primer sequences of PCR reaction
From: Lowering the n-6/n-3 PUFAs ratio inhibits the formation of THP-1 macrophage-derived foam cell
Gene | sequences | lengths | |
|---|---|---|---|
GAPDH | Forward | 5’-CACCCACTCCTCCACCTTTG-3’ | 110 bps |
Reverse | 5’-CCACCACCCTGTTGCTGTAG-3’ | ||
CD36 | Forward | 5’-AGTTGGAGACCTGCTTATC-3’ | 163 bps |
Reverse | 5’-GTTGCTGCTGTTCATCATC-3′ | ||
ABCA1 | Forward | 5’-TAACATCCTGCTTTGATTCC-3’ | 193 bps |
Reverse | 5’-TTGATTCTTTGCCTCTTACC-3’ | ||
ACAT1 | Forward | 5’-TGAAGGAAGTGGTCATAGTAAG-3’ | 246 bps |
Reverse | 5’-TGGTACATGGAGTAGAAATAGG-3’ | ||
LXRα | Forward | 5’-TCGGGCTTCCACTACAATGTTC-3’ | 234 bps |
Reverse | 5’-CTCTTGCCGCTTCAGTTTCTTC-3’ | ||
PPARγ | Forward | 5’-TCCACGAGATCATTTACAC-3’ | 155 bps |
Reverse | 5’-CTTCACAGCAAACTCAAAC-3’ |