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Fig. 3 | Lipids in Health and Disease

Fig. 3

From: Insulin promotes progression of colon cancer by upregulation of ACAT1

Fig. 3

ACAT1 siRNA significantly blocked the role played by insulin in tumor growth and metastasis. a: the effect of ACAT1siRNA on the inhibition of HT-29 cells proliferation in vitro in a CCK-8 assay, PBS as Blank group, Control siRNA as Control siRNA group. Data are representative of three CCK-8 assays independently performed, *P < 0.01 ACAT1 siRNA group vs. Blank group or Control siRNA group, **P < 0.01 Control siRNA + insulin group vs. Control siRNA group or ACAT1 siRNA + insulin group; b: the effect of ACAT1siRNA on the inhibition of HT-29 cells migration in vitro in a chemotaxis assay. Data are representative of three chemotaxis assays independently performed, *P < 0.05 ACAT1 siRNA group vs. Blank group or Control siRNA group, **P < 0.01 Control siRNA + insulin group vs. Control siRNA group or ACAT1 siRNA + insulin group; c: Equal doses of ACAT1 siRNA and control siRNA were used to transfer HT29 cells as indicated to inhibit ACAT1 expression. The data are expressed as the relative fold change. Data shown represent the averages of three replicates for each group analyzed via the 2-∆∆Ct method. *P < 0.01 ACAT1 siRNA group vs. Blank group or Control- siRNA group

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