Fig. 3

FOLE-induced cell death requires RIP1/3 complex and is licensed by caspase 8. a Assembly of RIP1/RIP3 complex and dissociation of caspase 8. IEC-6 cells were treated with 0.5% FOLE for 2–8 h. Whole cell extract was immunoprecipitated with anti-RIP1, and the immunoprecipitates were then immunoblotted with anti-RIP3 or caspase 8. b Representative immunoblots of caspase 8. The expression of caspase 8 was analyzed in the cells described above. c Caspase 8 activity. Cells were treated as described above. Data are presented as mean ± SD. **, p < 0.01 compared to control (n = 5–6 wells/group). d Cell viability. IEC-6 cells were pre-treated with Z-IETD-FMK (20 μM) for 40 min, followed by exposure to 0.25–0.5% FOLE for 2–12 h. Data are presented as mean ± SD (n = 6–8 wells/group). *, p < 0.05, **, p < 0.01 compared to FOLE (0.25%); #, p < 0.05 compared to FOLE (0.5%)