Fig. 2From: Toll-like receptor 2 activation primes and upregulates osteoclastogenesis via lox-1BMC surface expression of Lox-1 and RANK proteins. BMCs were cultured with or without Pam3CSK4 (TLR2 ligand, 100 ng/ml) or Lipid A (TLR4 ligand, 100 ng/ml) in the presence of M-CSF (20 ng/ml) for 3 days. Cells were washed with PBS and incubated with anti-Lox-1 or anti-RANK antibodies. Data are expressed as the mean ± SEM (n = 3 experiments)Back to article page