Fig. 3

Real time PCR to determine Lox-1, MyD88 and RANK expression in BMCs following addition of inhibitors. BMCs in the presence of M-CSF (20 ng/ml) were cultured with or without Pam3CSK4 (TLR2 ligand, 100 ng/ml) or Lipid A (TLR4 ligand, 100 ng/ml) for 3 days after incubation with TLR2/4 inhibitor (25 μM) or MAPK inhibitors, SP600125 (5 μM) as JNK inhibitor or U0126 (10 μM) as MEK inhibitor, respectively, for 1 h. a Both TLR2/4 inhibitor and MAPK inhibitors significantly suppressed TLR 2 or 4 ligand-induced upregulation of Lox-1 (upper panel) and MyD88 (lower panel) mRNA. b SP600125 significantly suppressed TLR 2, but not TLR 4 ligand-induced upregulation of RANK mRNA, and U0126 upregulated RANK mRNA. Data are expressed as the mean ± SEM (n = 3 experiments)