Fig. 4
From: Toll-like receptor 2 activation primes and upregulates osteoclastogenesis via lox-1
Effect of stimulation to TLRs and/or Lox-1 to TRAP-positive multinucleated cells. The effect of stimulation to Lox-1 with oxLDL and/or to TLR 2 with Pam3CSK4 was examined using TRAP staining. In each panel, E indicates stimulation at early-phase (BMC: day 0–3 before stimulation with RANKL) and L indicates stimulation at late-phase (BMMs: day 4–6 after stimulation with RANKL). (a and b upper) BMCs in the presence of M-CSF or BMMs in the presence of M-CSF and RANKL were cultured with oxLDL (30 μg/ml) at indicated phase. (a and b middle) BMCs in the presence of M-CSF or BMMs in the presence of M-CSF and RANKL were cultured with Pam3CSK4 (10 ng/ml) at indicated phase. (a and b lower) BMCs in the presence of M-CSF and Pam3CSK4 (25 ng/ml) were stimulated with oxLDL (15 μg/ml) at early- or late-phase. Data are expressed as the mean ± SEM (n = 3 experiments). *P < 0.05 compared with control