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Fig. 1 | Lipids in Health and Disease

Fig. 1

From: Naturally occurring 3RS, 7R, 11R-phytanic acid suppresses in vitro T-cell production of interferon-gamma

Fig. 1

Effects of naturally occurring phytanic acid (nPA) on interferon (IFN)-γ production and T-bet expression in mouse splenocytes. a Mouse splenocytes were incubated with palmitic acid or nPA, followed by an Alamar blue assay to determine cell viability. After incubation of phytohaemagglutinin (PHA)-stimulated splenocytes with palmitic acid or nPA, the concentrations of IFN-γ in culture supernatants (b), and mRNA expression of IFN-γ (c) and T-bet (d) were measured by ELISA and qRT-PCR, respectively. e Expression of IFN-γ mRNA was determined after PHA-stimulated splenocytes were incubated with nPA-bovine serum albumin (BSA) conjugate. The data represent means ± SEM. *P < 0.05, ** P < 0.01 and ***P < 0.001 compared to DMSO (dimethyl sulfoxide) control

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Lipids in Health and Disease

ISSN: 1476-511X