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Fig. 2 | Lipids in Health and Disease

Fig. 2

From: Naturally occurring 3RS, 7R, 11R-phytanic acid suppresses in vitro T-cell production of interferon-gamma

Fig. 2

Effects of naturally occurring phytanic acid (nPA) on interferon (IFN)-γ production in purified T-cells. a Immunomagnetically purified T-cells were incubated with nPA, followed by an Alamar blue assay to determine cell viability. After T-cells were incubated with nPA under phorbol 12-myristate 13-acetate (PMA)/ionomycin stimulation, IFN-γ mRNA expression (b) and concentrations of IFN-γ in culture supernatants (c) were measured by qRT-PCR and ELISA, respectively. The data represent means ± SEM. *P < 0.05 and ***P < 0.001 compared to dimethyl sulfoxide (DMSO) control

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