Fig. 5From: Combination of Chymostatin and Aliskiren attenuates ER stress induced by lipid overload in kidney tubular cellsCombination treatment with chymostatin and aliskiren markedly prevented ER stress and apoptosis in HK2 cells treated with cholersterol (10 μg /ml) for 6 days. a Protein abundance of ER stress markers (BiP, IRE1α, PERK, CHOP and p-eIF2α/eIF2α) were upregulated after cholesterol treatment, which was prevented by cotreatment with chymostatin (5X10−5M) and aliskiren (10− 8 M). b Quantitative analysis of ER stress markers levels normalized to β-actin. c The ratio of p-eIF2α/eIF2α. d Protein abundance of Bax and caspase-3 was upregulated after cholersterol treatment, which was prevented by cotreatment with chymostatin and aliskiren, whereas the level of Bcl-2 was unchanged. e The ratio of Bax and Bcl-2. f Quantitative analysis of cleaved-caspased 3 levels normalized to β-actin. Representative results of three independent experiments are shown. * p < 0.05 compared with controls; # p < 0.05 compared with chol. CTL, controls; chol, cholesterol treatment group; chol+CMT, cholesterol plus chymostatin treatment; chol+Ali, cholesterol plus aliskiren treatment; chol+CMT + Ali, cholesterol plus chymostatin and aliskiren treatmentBack to article page