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Fig. 1 | Lipids in Health and Disease

Fig. 1

From: Increased apolipoprotein M induced by lack of scavenger receptor BI is not activated via HDL-mediated cholesterol uptake in hepatocytes

Fig. 1

PCR-based genotyping analysis, confirmed by gel electrophoresis. a Curves of fluorescence (F) versus temperature (T) for sequence-specific base-quenched probe complementary to the SR-BI knockout gene sequence. b Derivative melting curves (-dF/dT vs. T) that depict the same data shown in panel A, wild-type (SR-BI+/+), heterozygous (SR-BI+/−), and homozygous (SR-BI−/−) mutant mice. c Two sets of primer pairs specific for the wild-type (primers 2 and 3) or targeted mutant (primers 2 and 4) alleles were used to screen genomic DNA by PCR as described. Representative results from wild-type (SR-BI+/+, lanes 3 and 4), heterozygous (SR-BI+/−, lanes 5 and 6), and homozygous mutant (SR-BI−/−, lanes 1 and 2) animals are shown

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Lipids in Health and Disease

ISSN: 1476-511X