Table 4 Primers used for RT-PCR
Primer | Primer Sequence (5′-3′) | Tm (°c) |
|---|---|---|
StAR | Forward: GCCCCGAGACTTCGTAAG Reverse: CAGGTGGGACCGTGTTCA | 59 |
P450scc | Forward: GCCCCGAGACTTCGTAAG Reverse: CAGGTGGGACCGTGTTCA | 60 |
3β-HSD | Forward: CCTGCTGCGTCCATTTTA Reverse: TCTGCTTGGCTTCCTCCC | 60 |
17β-HSD | Forward: ACCGCCGATGAGTTTGTT Reverse: GGGTGGTGCTGCTGTAGA | 60 |
β-Actin | Forward: GAGATTACTGCCCTGGCTCCTA Reverse: ACTCATCGTACTCCTGCTTGCTG | 60 |