Table 4 Primers used for RT-PCR
| Primer | Primer Sequence (5′-3′) | Tm (°c) |
|---|---|---|
| StAR |
Forward: GCCCCGAGACTTCGTAAG Reverse: CAGGTGGGACCGTGTTCA | 59 |
| P450scc |
Forward: GCCCCGAGACTTCGTAAG Reverse: CAGGTGGGACCGTGTTCA | 60 |
| 3β-HSD |
Forward: CCTGCTGCGTCCATTTTA Reverse: TCTGCTTGGCTTCCTCCC | 60 |
| 17β-HSD |
Forward: ACCGCCGATGAGTTTGTT Reverse: GGGTGGTGCTGCTGTAGA | 60 |
| β-Actin |
Forward: GAGATTACTGCCCTGGCTCCTA Reverse: ACTCATCGTACTCCTGCTTGCTG | 60 |
