Fig. 3
From: Intake of medium-chain fatty acids induces myocardial oxidative stress and atrophy
Heart morphology and expression of oxidative stress- and mitochondria-related proteins in cardiomyocytes. a and b Low- and high-power images of H&E staining in histological specimens, respectively. c-f Immunohistochemical staining for oxidative stress in nuclei (c) and cytoplasm (e) and graphs of the numbers of positive cells (d and f). Arrow indicates positive nucleus (c). g and h Immunohistochemical staining for mitochondria with a Letm1 antibody (g) and Letm1 expression quantified by western blotting (h). These data are expressed relative to the control, which was set as 100%. The data are expressed as the mean ± standard deviation. **p < 0.01, vs the control group; ***p < 0.001, vs the control group. H&E: hematoxylin and eosin; 8-OHdG: 8-hydroxy-2’-deoxyguanosine; 4-HNE: 4-hydroxy-2-nonenal; Con: control; Letm1: leucine zipper-EF-hand containing transmembrane protein 1