Table 2 Primer sequences
From: Trimethylamine-N-oxide (TMAO)-induced atherosclerosis is associated with bile acid metabolism
Gene | Forward and reverse primers | Gene | Forward and reverse primers |
|---|---|---|---|
Nr1h4 | f: 5′-TCCAGGGTTTCAGACACTGG-3′ r: 5′-GCCGAACGAAGAAACATGG-3′ | Baat | f: 5′-GGAAACCTGTTAGTTCTCAGGC-3′ r: 5′-GTGGACCCCCATATAGTCTCC-3′ |
Nr0b2 | f: 5′-CGATCCTCTTCAACCCAGATG-3′ r: 5′- AGGGCTCCAAGACTTCACACA-3′ | Slc27a5 | f: 5′-ACCCTGGATCAGCTCCTGGAT-3′ r: 5′-GTTCTCAGCTAGCAGCTTGG-3′ |
Cyp7a1 | f: 5′-AGCAACTAAACAACCTGCCAGTACTA-3′ r: 5′-GTCCGGATATTCAAGGATGCA-3′ | Abcc2 | f: 5′-GGATGGTGACTGTGGGCTGAT-3′ r: 5′-GGCTGTTCTCCCTTCTCATGG-3′ |
Cyp7b1 | f: 5′- TAGCCCTCTTTCCTCCACTCATA-3′ r: 5′-GAACCGATCGAACCTAAATTCCT-3′ | Abcc3 | f: 5′-TCCCACTTTTCGGAGACAGTAAC-3′ r: 5′-ACTGAGGACCTTGAAGTCTTGGA-3′ |
Cyp8b1 | f: 5′-GGCTGGCTTCCTGAGCTTATT-3′ r: 5′-ACTTCCTGAACAGCTCATCGG-3′ | Abcb11 | f: 5′-CTGCCAAGGATGCTAATGCA-3′ r: 5′-CGATGGCTACCCTTTGCTTCT-3′ |
Cyp27a1 | f: 5′-GCCTCACCTATGGGATCTTCA-3′ r: 5′-TCAAAGCCTGACGCAGATG-3′ | Slc10a1 | f: 5′-ATGACCACCTGCTCCAGCTT-3′ r: 5′-GCCTTTGTAGGGCACCTTGT-3′ |
18S | f: 5′-CAGGCATTGCTGACAGGATG-3′ r: 5′-TGCTGATCCACATCTGCTGG-3′ |  |  |