Skip to main content

Table 2 Primer sequences

From: Trimethylamine-N-oxide (TMAO)-induced atherosclerosis is associated with bile acid metabolism

Gene Forward and reverse primers Gene Forward and reverse primers
Nr1h4 f: 5′-TCCAGGGTTTCAGACACTGG-3′
r: 5′-GCCGAACGAAGAAACATGG-3′
Baat f: 5′-GGAAACCTGTTAGTTCTCAGGC-3′
r: 5′-GTGGACCCCCATATAGTCTCC-3′
Nr0b2 f: 5′-CGATCCTCTTCAACCCAGATG-3′
r: 5′- AGGGCTCCAAGACTTCACACA-3′
Slc27a5 f: 5′-ACCCTGGATCAGCTCCTGGAT-3′
r: 5′-GTTCTCAGCTAGCAGCTTGG-3′
Cyp7a1 f: 5′-AGCAACTAAACAACCTGCCAGTACTA-3′
r: 5′-GTCCGGATATTCAAGGATGCA-3′
Abcc2 f: 5′-GGATGGTGACTGTGGGCTGAT-3′
r: 5′-GGCTGTTCTCCCTTCTCATGG-3′
Cyp7b1 f: 5′- TAGCCCTCTTTCCTCCACTCATA-3′
r: 5′-GAACCGATCGAACCTAAATTCCT-3′
Abcc3 f: 5′-TCCCACTTTTCGGAGACAGTAAC-3′
r: 5′-ACTGAGGACCTTGAAGTCTTGGA-3′
Cyp8b1 f: 5′-GGCTGGCTTCCTGAGCTTATT-3′
r: 5′-ACTTCCTGAACAGCTCATCGG-3′
Abcb11 f: 5′-CTGCCAAGGATGCTAATGCA-3′
r: 5′-CGATGGCTACCCTTTGCTTCT-3′
Cyp27a1 f: 5′-GCCTCACCTATGGGATCTTCA-3′
r: 5′-TCAAAGCCTGACGCAGATG-3′
Slc10a1 f: 5′-ATGACCACCTGCTCCAGCTT-3′
r: 5′-GCCTTTGTAGGGCACCTTGT-3′
18S f: 5′-CAGGCATTGCTGACAGGATG-3′
r: 5′-TGCTGATCCACATCTGCTGG-3′