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Fig. 3 | Lipids in Health and Disease

Fig. 3

From: Simvastatin, but not pravastatin, inhibits the proliferation of esophageal adenocarcinoma and squamous cell carcinoma cells: a cell-molecular study

Fig. 3

Effect of simvastatin treatment on COX-2 expression in OE-19 and Eca-109 cells by study group. a OE-19 and Eca-109 cells were treated with 0.1% DMSO or different concentrations (15 μM, 30 μM, 45 μM, 60 μM and 75 μM) of simvastatin for 24 h. Total RNA was extracted for determination of mRNA levels of COX-2 with GAPHD as control. b OE-19 and Eca-109 cells were treated as indicated for 24 h. Total protein was extracted for immuoblotting of COX-2, using β-actin as loading control. The relative amounts of each protein were quantified by densitometry as ratios to β-actin. c Representative Western blot results for COX-2 and β-actin expression. * indicates significant difference from DMSO group (P < 0.05). COX-2, Cyclooxygenase-2; Eca-109, Esophageal squamous cell carcinoma; OE-19, esophageal adenocarcinoma; DMSO, dimethylsulfoxide; TBA, thiobarbituric acid

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Lipids in Health and Disease

ISSN: 1476-511X