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Fig. 2 | Lipids in Health and Disease

Fig. 2

From: κ-Opioid receptor stimulation reduces palmitate-induced apoptosis via Akt/eNOS signaling pathway

Fig. 2

U50,488H activated Akt/eNOS pathway and enhanced NO production. HUVECs were treated with vehicle, palmitate (450 μmol/L), U50,488H (70 μmol/L), Nor-BNI (10 μmol/L), LY-294002 (20 μmol/L), MK-2206-2HCl (0.2 μmol/L), and L-NAME (100 μmol/L) for 48 h. a-b The expression and phosphorylation of Akt and eNOS were determined by Western blot. c The NO production was assayed as methods described. d The expression of caspase-3 was determined by Western blot. Values are mean ± SEM. n = 6. High fat: sodium palmitate, U50: U50,488H, nor-BNI, nor-binaltorphimine. LY294002, MK2206-HCl, L-NAME were specific inhibitors to PI3K, Akt and eNOS, respectively. *P < 0.05 vs. Control, ##P < 0.01 vs. High fat, $$P < 0.01 vs. High fat +U50

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