Fig. 3

U50,488H inhibited high fat-induced HUVECs apoptosis in a PI3K/Akt/eNOS dependent manner. Cultured HUVECs were transfected with scramble, κ-OR, Akt siRNA for 12 h. a-b After the transfection, the expression of κ-OR and Akt were determined by Western blot. c-g In scramble, κ-OR or Akt siRNA-transfected HUVECs, sodium palmitate (450 μmol/L) and U50,488H (70 μmol/L) were added for 48 h. The expression of κ-OR, Akt, phosphorylated Akt, eNOS and phosphorylated eNOS were determined by Western blot. Values are means±SEM. n = 4. High fat: sodium palmitate, U50: U50,488H, ^*P < 0.05, ^**P < 0.01 vs. Control, ^#P < 0.05, ^##P < 0.01 vs. High fat, ^$P < 0.05, ^$$P < 0.01 vs. High fat +U50 + scramble siRNA1, ^&P < 0.05, ^&&P < 0.01 vs. High fat +U50 + scramble siRNA2