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Fig. 4 | Lipids in Health and Disease

Fig. 4

From: κ-Opioid receptor stimulation reduces palmitate-induced apoptosis via Akt/eNOS signaling pathway

Fig. 4

U50,488H regulated pro- and anti-apoptotic protein expression through PI3K/Akt/eNOS signaling pathway. Cultured HUVECs were transfected with scramble, κ-OR, Akt siRNA for 12 h. In scramble, κ-OR or Akt siRNA-transfected HUVECs, sodium palmitate (450 μmol/L) and U50,488H (70 μmol/L) were added for 48 h. a-c The expressions of caspase-3, Bax, and Bcl-2 were determined by Western blot. Values are means±SEM. n = 4. High fat: sodium palmitate, U50: U50,488H. **P < 0.01 vs. Control, ##P < 0.01 vs. High fat, $$P < 0.01 vs. High fat +U50 + scramble siRNA1, &P < 0.05, &&P < 0.01 vs. High fat +U50 + scramble siRNA2

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