Table 1 Primers of the RT-qPCR
From: Double bonds of unsaturated fatty acids differentially regulate mitochondrial cardiolipin remodeling
Gene | Accession Number | Forward primer 5′-3′ | Reverse primer 5′-3′ |
|---|---|---|---|
Gapdh | NM_008084 | AACTTTGGCATTGTGGAAGG | GGATGCAGGGATGATGTTCT |
Cds1 | NM_173370 | GGAGAGACGGTGGCAGATTA | CAGGTAGAGGGCGAAGGATA |
Pgs1 | NM_133757 | ACGCTGATTGGCTCTCCTAA | CTGCTCTTGCTCCTGATGAA |
Lclat1 | NM_001081071 | ACCGCCTAAGAGAAGGGAAG | TGGATGTGGGAAGAGAGTCA |
Taz | NM_​001173547 | CCTTATCACCGTCTCCAACC | GTCCAACGCATCAACTTCAG |
Pla2g6 | NM_001199023 | GAGACTGCCTTCCATTACGC | TCAGCCCTTGGTTGTTTACC |
Pnpla8 | NM_26164 | TTCCTTTCTCGTCCCACTGA | GCAGACACTTCCTGTTCTTCG |
Fads2 | NM_019699 | CAAAACCAACCACCTGTTCTT | AAAGGCTGTGACGAGGGTAG |
Pld6 | NM_001290283 | CTCTGCCTCTTCGCCTTCT | ACCTGTATCCCTGCCTTGC |
Cycs | NM_007808 | AAATCTCCACGGTCTGTTCG | TGCCCTTTCTCCCTTCTTCT |
Bid | NM_007544 | AGCCCTTGATGAGGTGAAGA | GCAAAGATGGTGCGTGACT |
Plscr3 | NM_001168497 | CTGGACTTGTGGCTGTGGTA | GGCATCTGTGAGGGCTTCT |
Ptgs1 | NM_008969 | ACAGTGCGGTCCAACCTTAT | AGAGGGCAGAATGCGAGTAT |
Ptgs2 | NM_011198 | CCCCCACAGTCAAAGACACT | ATCATCAGACCAGGCACCA |
Alox5 | NM_009662 | CATCAAGAGCAGGGAGAAGC | CATAGTTGGAGGAGCGTTGG |
Fads2 | NM_019699 | CAAAACCAACCACCTGTTCTT | AAAGGCTGTGACGAGGGTAG |
Cpt1a | NM_013495 | ATGGACTCTAGTGATACA | ATCTCTGGATGTAGTAGG |
Cpt2 | NM_009949 | CCAGTTCAGGAAGACAGA | CGAGCAGTTAAATACATATCAAA |