Fig. 2
From: LncRNA MALAT1 modulates ox-LDL induced EndMT through the Wnt/β-catenin signaling pathway
Changes of cellular morphology, EndMT markers and MALAT1 in HUVECs. HUVECs were treated with ox-LDL at different concentrations (10, 20, 40 μg/ml) for 48 h. a Cellular F-actin was stained with rhodamine phalloidin. Nuclei were stained with DAPI Fluoromount-G®. Cells were observed under fluorescence microscopy. Magnification, 400x. b-d Endothelial markers (CD31, vWF) and mesenchymal markers (α-SMA, vimentin) were detected by qRT-PCR (B), Western blot (C) (n = 3, *P < 0.05, **P < 0.01, versus control group) and immunofluorescence analysis (D). Magnification, 400x. e MALAT1 expression was detected by qRT-PCR (n = 3, *P < 0.05, **P < 0.01 versus control group). The relative levels of α-SMA, vimentin, CD31, vWF and MALAT1 in ox-LDL groups were normalized to those in control group, respectively. f Pearson correlations between MALAT1 and α-SMA mRNA, vimentin mRNA, CD31 mRNA, vWF mRNA