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Fig. 3 | Lipids in Health and Disease

Fig. 3

From: LncRNA MALAT1 modulates ox-LDL induced EndMT through the Wnt/β-catenin signaling pathway

Fig. 3

Knockdown of MALAT1 attenuates ox-LDL-induced EndMT in HUVECs. a HUVECs were transfected with MALAT1-siRNA1, MALAT1-siRNA2, MALAT1-siRNA3 or scramble control (scr) for 24 h. The MALAT1 levels were determined by qRT-PCR (n = 3, *P < 0.05, versus negative control group). b The effect of MALAT1-siRNA on cell morphology induced by ox-LDL (40 μg/ml) was observed through rhodamine phalloidin-stained F-actin. Magnification, 400x. c-e The effect of MALAT1-siRNA3 on the expression of endothelial markers (CD31, vWF) and mesenchymal markers (α-SMA, vimentin) were detected by qRT-PCR (c), Western blot (d) (n = 3, *P < 0.05, **P < 0.01 versus control group, #P < 0.05, ##P < 0.01 versus ox-LDL group) and immunofluorescence analysis (e). Magnification, 400x

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