Fig. 4
From: LncRNA MALAT1 modulates ox-LDL induced EndMT through the Wnt/β-catenin signaling pathway
The effect of MALAT1 overexpression on EndMT of HUVECs. Full-length human MALAT1 cDNA plasmid (pcDNA-MALAT1) was constructed and transfected into HUVECs. The empty vector was used as negative control. a MALAT1 expression was detected by qRT-PCR (n = 3, *P < 0.05, versus pcDNA vector group). b The effect of MALAT1 overexpression on cell morphology induced by ox-LDL (40 μg/ml) was observed through rhodamine phalloidin-stained F-actin. Magnification, 400x. c-e The effect of MALAT1 overexpression on the expression of endothelial markers (CD31, vWF) and mesenchymal markers (α-SMA, vimentin) were detected by qRT-PCR (c), Western blot (d) (n = 3, *P < 0.05, **P < 0.01 versus pcDNA vector group) and immunofluorescence analysis (e). Magnification, 400x