Fig. 2

Mutations of the modified alleles were detected by sequencing. (a) Mutations generated by LDLR sgRNA 2, 4 and apoE sgRNA 5, primer P1, P2, P3 were used (1♂, 2♀, 4♂). (b) Mutations generated by LDLR sgRNA 1, 3 and apoE sgRNA 6, primer P1, P2, P3 were used (5♀, 6♂). (c) Mutations generated by LDLR sgRNA 2,4 and apoE sgRNA 5 (3♀) and LDLR sgRNA 1–4 (7♂), primer P4 were used. WT refers to the wild-type allele sequences, deletions (−), insertions(+)and substitutions (Δ) are shown, target sequences are red. No mutation was found in 1♂ and the sgRNA 3, 3♀ and 7♂ showed the large-fragment deletion